A method of culturing mesenchymal stem cells is provided. The mesenchymal stem cells are cultured in a medium that includes a cell-depleted Whartons Jelly filtrate. The cell-depleted Whartons Jelly filtrate is prepared by mincing umbilical cord tissue that includes Whartons Jelly, subsequently diluting the umbilical cord tissue, subsequently filtering the umbilical cord tissue to generate a filtrate, and sedimenting Whartons Jelly stem cells from the filtrate to generate the cell-depleted Whartons Jelly filtrate.
