HAYES, BRADLEY,BEAM, KEVIN,MEYER, DAMON,LYON, ROBERT,VALLIERE-DOUGLASS, JOHN
申请号：
CA2928238
公开号：
CA2928238A1
申请日:
2014.11.21
申请国别(地区):
CA
年份:
2015
代理人:
摘要：
The invention is based in part on the observation that a CHO cell oxidizing enzyme, particularly QSOX1, can survive a seemingly rigorous antibody purification process to reduce subsequent conjugation efficiency of the antibody to a drug. Whether the oxidizing enzyme survives the purification procedure depends on which purification techniques are employed which can vary from one antibody to another. With knowledge that contamination with a CHO cell oxidizing enzyme is a potential problem for subsequent conjugation, a suitable purification scheme can be devised for any antibody that eliminates or at least reduces CHO oxidizing enzyme(s) to an acceptable level.
