A Light Emitting Diode (LED) light source control system for intelligentized layer breeding, comprises a plurality of LED lamps (30) equipped at a layer breeding place and a LED light source controller (10). Each of the LED lamps (30) comprises a red light LED and a green light LED. The LED light source controller (10) controls the LED lamps (30) to emit green light during the brooding period and the improved period of a layer; and the LED light source controller (10) controls the LED lamps (30) to emit red light during the egg producing period of a layer. The LED light source controller (10) controls the illumination intensity of the LED lamps (30) by regulating the duty ratio of an output Pulse-Width Modulation (PWM). The LED light source controller (10) controls the illumination time of the LED lamps (30) by utilizing a clock unit so as to implement different illumination time respectively during various stages of the brooding period, the improved period and the egg producing period of a layer. The system can improve the production performance of the layer effectively, realize the purpose of increasing production and emission reduction, and decrease the mortality of the layer.