Provided is a micro-fluidic device (1000) for enriching circulating tumor cells including a body (100), defining a fluid flowing chamber, the fluid flowing chamber having an opening at an upper side thereof; an inlet (200), disposed at a bottom of the body (100); an outlet (300), disposed at the bottom of the body (100); a plasma fluorescence enhancement chip (400), disposed at the opening of the fluid flowing chamber, and loaded with antibodies at a lower surface thereof; and a magnetic field generating component (500), disposed at an upper surface of the plasma fluorescence enhancement chip (400). Also provided are a kit and a system including the micro-fluidic device (100), a method for enriching circulating tumor cells, and a method for detecting circulating tumor cells.
