formulation for treating cells expressing hcv proteins and hepatitis c carriers, method for treating cells infected with the hcv genotype 2a pj6 / jfh1 strain and use of a formulation
THE CHARLOTTE-MECKLENBURG HOSPITAL AUTHORITY D/B/A CAROLINAS MEDICAL CENTER
发明人:
HERBERT L. BONKOVSKY,WEIHONG HOU
申请号：
BRPI0914060
公开号：
BRPI0914060A2
申请日:
2009.10.08
申请国别(地区):
BR
年份:
2015
代理人:
摘要：
The present invention is directed to methods of treating cells infected with HCV and mammals suffering from HCV infection by transfecting the infected cells with miRNA-196 mimic. miRNA-196 mimic significantly down-regulates Bach1 protein and HCV gene expression, while also up-regulating HMOX1 gene expression. miRNA-196 binds with the 3'-UTR of Bach1 mRNA to reduce the expression of Bach1. As such, miRNA-196 can play an important role in the regulation of HCV replication and HMOX1/Bach1 expression in hepatocytes. The present invention also provides a formulation for the treatment of cells expressing HCV comprising a therapeutically effective amount of miRNA-196 such that Bach1 and HCV gene expression are down- regulated while HMOX1 expression is increased. The formulations are adapted to enable the transfection of miRNA-196 mimic into hepatocytes expressing HCV proteins.
