PROBLEM TO BE SOLVED: To provide anti-biotin antibodies and anti-biotin derivative antibodies capable of binding biotin with sufficient affinity such that the antibodies are useful as diagnostic and/or therapeutic agents in targeting biotin.SOLUTION: The invention provides: a humanized anti-biotin antibody comprising a heavy or light chain hypervariable region (HVR) comprising a specific amino acid sequence; a monoclonal antibody of the antibody, or an antibody fragment that binds biotin; a pharmaceutical formulation comprising the antibody and a pharmaceutically acceptable carrier; and a method of using the antibody.SELECTED DRAWING: Figure 2【課題】抗体が診断剤および/または治療剤としてビオチンのターゲティングに役立つように、十分な親和性でビオチンと結合する能力を有する、抗ビオチン抗体および抗ビオチン誘導体抗体の提供。【解決手段】特定のアミノ酸の配列を含む重鎖または軽鎖の超可変領域(HVR)を含む、ヒト化抗ビオチン抗体、該抗体のモノクローナル抗体またはビオチンと結合する抗体フラグメント、該抗体と薬学的に許容される担体とを含む薬学的製剤、および該抗体の使用方法。【選択図】図2