The application relates to a device for drawing and/or holding bodily fluids, in particular blood, comprising a tube (1), which is tightly closed at the upper, open end thereof by means of a cap (3), which is designed to have a non-return valve (11) and has a dome-like attachment (4) in order to hold a cannula or double cannula (7) carried by a needle holder (8), which attachment holds a membrane (9) at the upper end of the attachment in a longitudinal bore (5), which membrane can be pierced by the cannula. In order that the effectiveness of the non-return valve and the operational reliability can be improved, the non-return valve according to the invention is arranged in the dome-like attachment together with a holding means (13) provided in the longitudinal bore below the membrane that can be pierced, wherein the holding means is designed to have at least one through-flow opening (18), which can be closed to the tube interior by the non-return valve after the fluid draw.
