Chip, system and method using microfluidic devices and instrumentation for multiple parameter stimulation and recording on cells or organs is presented. The chip consists of multilayer fluidic channels to organize cells or organs and to flow stimulant molecules or cells. Further an array of such channels are arranged spatially for multiple biochemical reaction or assay or co-culture. The reactors are also arranged in one or more spiral fluidic channel with multiple connecting channels between two or more such spiral channels. The system consists of stimulation instrumentation for the cells or organs using optical, electrical, mechanical, fluidic and chemical and recording instrumentation for signals or images from the cells simultaneously or alternatively are performed using optical imaging, electrical field potential and impedance. Such system is operated remotely from an incubator or microscopic sterile environment using wireless or wired networks. The methodology for performing assay and drug screening uti
