The present invention discloses a bio-fiber membrane having a particulate storage layer and a functional particle channel, comprising a bio-fiber body having a first surface and a second surface opposite the first surface, and a plurality of the bio-fibers disposed on the bio-fiber a functional particle channel inside the body and extending through the first surface and the second surface; a functional particle storage layer disposed on the first surface of the biofiber body and containing a plurality of functional particles; The functional particles are a plurality of Leigong root extracts, a plurality of Irish algae extracts, a plurality of okra extracts and a plurality of aloe extracts, and have a size smaller than an inner diameter of the functional particle channel.本創作揭示一種具有微粒儲存層與機能微粒通道的生物纖維膜片,包含一生物纖維本體,具有一第一表面及與第一表面相背的一第二表面,以及複數個設置於該生物纖維本體內部並貫通該第一表面及該第二表面的機能微粒通道;一機能微粒儲存層,該機能微粒儲存層設置於該生物纖維本體之第一表面上並包含有複數個機能微粒;其中,該些機能微粒複數個雷公根萃取物,複數個愛爾蘭藻萃取物,複數個秋葵萃取物及複數個蘆薈萃取物,並具有小於該機能微粒通道內徑之尺寸。