РАЙ Гиан П. (US),РОСАЛЕС Франциско Дж. (SG),ДЖОУНИ Зейна Э. (US),ВАВОРУНТУ Розалин (US)
申请号：
RU2011120789/15
公开号：
RU2011120789A
申请日:
2009.10.23
申请国别(地区):
RU
年份:
2012
代理人:
摘要：
1. A method for determining the biological activity of factor TGF-β in a sample of a powdered food composition or a powdered source of untreated protein, comprising: a. Restoring the sample to a concentration of from about 140 mg / ml to about 150 mg / ml； b. Centrifuging the reconstituted sample at approximately 10,000 rpm for approximately 10 minutes and maintaining the supernatant layer； c. Acidification of the supernatant layer to a pH of from about 2 to about 3； d. Incubation of the supernatant layer for approximately 15 minutes at room temperature； e. Centrifuging the supernatant layer at about 10,000 rpm for about 10 minutes and maintaining the supernatant layer f. Neutralization of the supernatant layer from step (e) to a pH of from about 7 to about 7.5； g. Centrifuging the supernatant layer from step (f) at about 10,000 rpm for about 10 minutes and maintaining the supernatant layer h. Contacting the supernatant layer with HT-2 and i cells. Determination of the concentration at which the inhibition of biological activity in HT-2 cells is 50%. 2. The method according to claim 1, wherein the food composition is selected from the group consisting of a food additive, a baby food product, a mixture for feeding infants and a fortifier for breast milk. The method according to claim 1, wherein the acidification step comprises adding concentrated HCl. The method according to claim 1, where the stage of neutralization includes the addition of 50% NaOH. A method for determining the biological activity of factor TGF-β in a sample of liquid milk, comprising: a. Centrifuging the sample at approximately 13,000 rpm for approximately 151. Способ определения биологической активности фактора TGF-β в образце порошкообразной пищевой композиции или порошкообразного источника необработанного белка, включающий:a. Восстановление образца до концентрации от приблизительно 140 мг/мл до приблизительно 150 мг/мл；b. Центрифугирование восстановленного образца при приблизительно 10000 об/ми
