Tetsuya YAMAGATA,Yuanbo QIN,Haruhiko MORITA,Talha AKBULUT,lain Robert THOMPSON
申请号：
US16483997
公开号：
US20190376088A1
申请日:
2018.02.07
申请国别(地区):
US
年份:
2019
代理人:
摘要：
The present invention provides a method of treating a disease associated with elevated KRAS activity or expression in a subject, comprising suppressing KRAS expression in the subject by targeting an expression regulatory region of KRAS gene using a CRISPR-Guide Nucleotide Directed Modulation (GNDM). Also, provided is a CRISPR-GNDM system for suppressing KRAS expression comprising (a) a protein selected from the group consisting of dCas9 or dCpf1, a fusion protein of dCas9 or dCpf1 and Kruppel associated box (KRAB), and (b) a guide nucleotide targeting an expression regulatory region of KRAS gene.
