The present invention provides a method for processing cultured cells. The method includes the steps of providing a biochamber for culturing cells； providing a culture media for culturing cells within the biochamber； inoculating the biochamber with cells； culturing the cells； upon a predetermined time period of culture, displacing the culture media from the biochamber with a biocompatible first rinse solution； replacing the first rinse solution with a cell harvest enzyme solution； incubating the contents of the biochamber for a predetermined period of time, wherein during incubation, the enzyme at least dissociates the cells from each other and/or from the biochamber surface； and displacing the enzyme solution with a second rinse solution, wherein upon the enzyme being displaced, the chamber is substantially filled with the second rinse solution.
