KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
发明人:
CHO, YEE SOOK,조이숙,LEE, KYEONG,LEE, JUNG WOON,이정운
申请号：
KR1020120126352
公开号：
KR1020130133638A
申请日:
2012.11.08
申请国别(地区):
KR
年份:
2013
代理人:
摘要：
According to the present invention, when novel low molecular weight compound RSC-133 is added during the reprogramming inducing culture for preparing pluripotent stem cells in human differentiated cells, the reprogramming inducing efficiency can be improved and the time for reprogramming induction can be significantly reduced. Particularly, it was verified that RSC-133 was substitutable for c-Myc which is a reprogramming inducing factor and a cancer-causing risk factor, and effectively improved the reprogramming inducing efficiency in both normal oxygen culture conditions and hypoxia culture conditions. Further, it was verified that RSC-133 inhibited the induction of aging, which occurs during the reprogramming inducing procedure, promoted the slowdown of cell proliferation, and induced epigenetic gene activation to improve reprogramming inducing culture conditions. The present invention contributes to the optimization of the procedure for preparing pluripotent stem cells from a small amount of patient-specific somatic cells secured from various sources, thereby remarkably improving the development of clinically applicable cell therapy products of personalized stem cells and the development of new drugs and contributing to the acceleration of commercialization thereof. According to another aspect of the present invention, it was verified that novel low molecular weight compound RSC-133 would be a culture medium composition that effectively maintains the undifferentiated state of human embryonic stem cells, which are representative pluripotent stem cells. The medium composition added with RSC-133 can effectively induce the undifferentiated proliferation of human embryonic stem cells, and are useful in developing the mass culture system of embryonic stem cells.COPYRIGHT KIPO 2014본 발명에 의하면, 인간 분화세포에서 다능성 줄기세포를 제작하기 위한 역분화 유도 배양과정에 신규한 저분자 화합물 RSC-133을 첨가하면, 역분화 유도 효율을 증진시킬 수 있을 뿐만 아니라 역분화 유도에 소요되는 시간을 획기적으로 줄일 수 있다. 특히, 역분화 유도인자이자 암유발 위험인자인 c-Myc을 대체할 수 있는 것으로 확인되었으며,
