Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogs, kits comprising the compounds, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods for making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.L'invention concerne des analogues de coelentérazine de formule (I), des procédés de fabrication de ces analogues, des trousses comprenant lesdits analogues, et des procédés d'utilisation de ces composés pour la détection de luminescence dans des dosages à base de luciférase.
