Method for inducing differentiation from intermediate mesoderm cells to renal progenitor cells, and method for inducing differentiation from pluripotent stem cells to renal progenitor cells
A method for producing renal progenitor cells from pluripotent stem cells, comprising the following steps (i) to (vi). (I) culturing the pluripotent stem cells in a medium containing FGF2, BMP4, a GSK-3β inhibitor and retinoic acid or a derivative thereof； (ii) converting the cells obtained in step (i) into FGF2, GSK- (Iii) a step of culturing in a medium containing 3β inhibitor and BMP7； (iii) a step of culturing the cells obtained in step (ii) in a medium containing FGF2, GSK-3β inhibitor, BMP7 and TGFβ inhibitor； ) Culturing the cells obtained in step (iii) in a medium containing FGF2, a GSK-3β inhibitor, BMP7, activin and a ROCK (Rho-kinase) inhibitor； (v) obtaining in step (iv) Culturing the cells obtained in a medium containing retinoic acid or a derivative thereof and FGF9； and (vi) transforming the cells obtained in step (v) with a GSK-3β inhibitor and FGF Culturing in a medium containing No. 9 to induce renal progenitor cells from intermediate mesoderm cells.多能性幹細胞から腎前駆細胞を製造する方法であって、以下の工程（ｉ）～（ｖｉ）を含む、方法。（ｉ）多能性幹細胞を、ＦＧＦ２、ＢＭＰ４、ＧＳＫ－３β阻害剤およびレチノイン酸またはその誘導体を含む培地で培養する工程；（ｉｉ）工程（ｉ）で得られた細胞を、ＦＧＦ２、ＧＳＫ－３β阻害剤およびＢＭＰ７を含む培地で培養する工程；（ｉｉｉ）工程（ｉｉ）で得られた細胞を、ＦＧＦ２、ＧＳＫ－３β阻害剤、ＢＭＰ７およびＴＧＦβ阻害剤を含む培地で培養する工程；（ｉｖ）工程（ｉｉｉ）で得られた細胞を、ＦＧＦ２、ＧＳＫ－３β阻害剤、ＢＭＰ７、アクチビンおよびＲＯＣＫ（Rho-kinase）阻害剤を含む培地で培養する工程；（ｖ）工程（ｉｖ）で得られた細胞を、レチノイン酸またはその誘導体およびＦＧＦ９を含む培地で培養する工程；および（ｖｉ）工程（ｖ）で得られた細胞を、ＧＳＫ－３β阻害剤およびＦＧＦ９を含む培地で培養し、中間中胚葉細胞から腎前駆細胞を誘導する工程。
