The present invention provides a fiber-weaving hygiene item and the fabricating method thereof. The hygiene item comprises a substrate, a fabric-base fiber, and a sensing matter. The fabricating method comprises the following steps: preparing a substrate, forming a plurality of penetrating holds on the substrate, preparing a fabric-base fiber and interlacing the fabric-base fiber on a first surface and a second surface of the substrate, and disposing a sensing matter on the fabric-base fiber, wherein the hydrophilicity of the fabric-base fiber is higher than that of the substrate. The present invention applies the characteristic of the higher hydrophilicity of the fabric-base fiber for luring a matter under test toward the fabric-base fiber. When the matter under test is contacted with the fabric-base fiber, the sensing matter can change its color for bio-examination. Using the present invention, multiple bio-examinations can be done for examining the matter under test.
