JONES, Christopher D.,QUINTANAR, Nathan,PATEL, Roshan
申请号：
EP17813877
公开号：
EP3468570A4
申请日:
2017.06.12
申请国别(地区):
EP
年份:
2020
代理人:
摘要：
A method of making a composition for influencing biological growth includes obtaining a sample comprising human umbilical cord blood and having a first volume VUC, combining the sample with a quantity of PrepaCyte-CB or the equivalent having a volume VPC, wherein the ratio VUC/VPC is between about 0.60 and about 1.75 such that a supernatant is formed, layering within a container at least a portion of the supernatant over a layer of a reagent configured for isolating mononuclear cells, the at least a portion of the supernatant having a volume VS and the layer of the reagent having a volume VR, wherein the volume of the layer of the reagent VR is at least 67% the volume VS of the at least a portion of the supernatant, placing a sufficient centrifugal force on the contents of the container to cause at least a new layer substantially comprising mononuclear cells to form, combining at least some of the layer substantially comprising mononuclear cells with lactated Ringer's solution or the equivalent to create a mononuclear cell solution, determining the total number of live cells NL in at least a portion of the mononuclear cell solution, the at least a portion of the mononuclear solution having a volume VM, and adding a volume VD of Low Molecular Weight Dextran in Dextrose solution to the at least a portion of the mononuclear cell solution, the volume VD based at least in part on the total number of live cells NL.
