A method of measuring the effect of an active agent or a stressor on the baseline metabolic indicators of one or more hair follicles, the method including (a) obtaining one or more hair follicles (b) placing the one or more hair follicles in a vessel, wherein the one or more hair follicles are positioned under one or more sensors (c) using the one or more sensors to measure the one or more hair follicles baseline metabolic indicators for both glycolysis and oxidative phosphorylation (d) exposing the one or more hair follicles to an active agent or a stressor and (e) using the one or more sensors to measure the one or more hair follicles respondent metabolic indicators for both glycolysis and oxidative phosphorylation.
