A method and composition for providing the formulation of Efavirenz or Tenofovir or Dapivirine and Curcumin loaded Lactoferrin Nano-particles (ECNPs) to treat Sexually Transmitted Infections (STI™s), and STI-mediated inflammation, contraception in vagina is disclosed. The method includes the step of formulating nanoparticle of 40 milligram Lactoferrin loaded with 20 milligram of 94 percent pure Curcumin and 10 milligram of 98 milligram pure Efavirenz or Tenofovir or Dapivirine by sol-oil method. The method further includes the step of dissolving Lactoferrin in 500 microliter ice cold Phosphate Buffered Saline (PBS) having a pH of 7.4.Then the method includes the step of dissolving Curcumin and Efavirenz in 100 microliter of Dimethyl Sulfoxide (DMSO). The method further includes the step of adding the dissolved Curcumin and Efavirenz or Tenofovir or Dapivirine to the dissolved Lactoferrin and adding ice cold olive oil. Protein aggregation at this interface is prevented by sonication followed by exposure to low temperature resulting in pellets which are precipitated and washed to provide ECNPs or TCNPs or DCNPs.
