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PROCÉDÉ DE PRODUCTION DE CELLULES PRÉCURSEURS NEURALES PRODUISANT DE LA DOPAMINE
专利权人:
Kyoto University;Sumitomo Dainippon Pharma Co., Ltd.
发明人:
TAKAHASHI, Jun,DOI, Daisuke,YOSHIDA, Kenji,KUWAHARA, Atsushi,TAKAHASHI, Masayo
申请号:
EP17786067
公开号:
EP3447130A4
申请日:
2017.04.24
申请国别(地区):
EP
年份:
2019
代理人:
摘要:
A cell population comprising Corin- and/or Lrtml-positive cells was produced by the following steps (1) and (2), from which Corin positive and/or Lrtml positive cells are collected using a substance that binds to Corin and/or a substance that binds to Lrtml, and dopaminergic neuron progenitor cells are produced by performing suspension culture of the Corin positive and/or Lrtml positive cells in a culture solution containing one or more nutritional factors: (1) a step of performing adhesion culture of pluripotent stem cells in a medium for maintaining undifferentiated state containing a Sonic hedgehog (SHH) signal stimulant, and an undifferentiated state-maintaining factor in the absence of feeder cells but in the presence of an extracellular matrix, and (2) a step of culturing the cell population obtained in the step (1) in a culture solution containing one or more differentiation-inducing factors.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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