Michael S. Kinch,Michael Goldblatt,Wu-Bo Li,Douty Bamba,Shaojing Chang,Huosheng Chen,Zenbework Fesseha,Manu Kohli,Hanwen Mao,Heather Thi Thu Ung-Medoff,Ke Weng
申请号：
US12652877
公开号：
US08535684B2
申请日:
2010.01.06
申请国别(地区):
US
年份:
2013
代理人:
摘要：
A method for identifying host genes and encoded proteins for potential targets for therapeutic intervention employs a Gene Search Vector that is either lentivirus or MMLV-based, and can be used to interrogate an entire cell genome without prior knowledge of the genomic sequence. This Random Homozygous Gene Perturbation (RUGP) technique is rapidly verifiable and is used to identify potential host targets for intervention for influenza, HIV and other viral infections. Using Thermal Assymetric Interlaced (TAIL)-PCR, the period for identification of promising targets is reduced from months to weeks or less. Specific targets including PTCH1, Robo1 and Nedd4 are reviewed in detail.
