Disclosed are a protein associated with disease resistance and an encoding gene thereof, and use thereof in regulation of plant disease resistance. Proteins provided in the present invention are proteins in a) or b) or c) or d) as follows: a) an amino acid sequence is a protein of amino acids 1-264 of sequence 1; b) the amino acid sequence shown as amino acids 1-264 of sequence 1 in the sequence listings is subjected to substitutions and/or deletions and/or additions of one or several amino acid residues to obtain a protein associated with disease resistance; c) the amino acid sequence is the protein of sequence 1; d) the amino acid sequence shown as sequence 1 in the sequence listings is subjected to substitutions and/or deletions and/or additions of one or several amino acid residues to obtain a protein associated with disease resistance. Experiments demonstrate that the protein associated with disease resistance and the encoding gene thereof of the present invention can be used to enhance plant disease res
