The invention relates to a process for the purification of hyperglocysylated analogue of erythropoietin. A process comprising of at least four chromatographic purification steps i.e. at least two anion exchange chromatographies, at least one hydroxyapatide chromatography and atleast one gel permeation chromatography. In a preferred embodiment, this process does not require any affinity chromatography and any reverse phase chromatography. The invention relates to, in particular, a process wherein the following chromatographic purification steps have the following order: a) a first anion exchange chromatography b) a hydroxyapatite chromatography c) a second anion exchange chromatography d) a gel permeation chromatography.
