The present invention relates to a methodfor polypeptide transfer into cells. The present inventionfurther relates to the detection of polypeptide-specificimmune cells and the priming, expansion and reactivation ofpolypeptide-specific T cells. Moreover the present inventionrelates to polypeptides of the methods of the presentinvention in combination with urea and their use for research,diagnosis or treatment and prevention of diseases inanimals and humans.