polypeptide fragment, isolated polynucleotide, recombinant vector, recombinant host cell, method for identifying compounds, compound, antibody, and use of a compound, pharmaceutical composition, or antibody
polypeptide fragment, isolated polynucleotide, recombinant vector, recombinant host cell, methods for identifying compounds and for producing compounds, antibody and use thereof the present invention relates to polypeptide fragments comprising an amino terminal fragment of the pa subunit of an rna viral RNA-dependent polymerase, which has endonuclease activity, in which said pa subunit is from the influenza to pandemic virus h1n1 2009 or is a variant thereof. this invention also relates to (i) crystals of the polypeptide fragments that are suitable for determining the structure of said polypeptide fragments using x-ray crystallography, and (ii) computational methods that use the structural coordinates of said polypeptide to selecting and determining compounds that modulate, preferably inhibit, the endonucleolytically active site in the polypeptide fragment. moreover, this invention relates to methods for identifying compounds that bind to polypeptide fragments pa, which have endonuclease activity, and preferably inhibit said endonucleolytic activity, preferably in an environment of high productivity. this invention also relates to compounds that are capable of modulating, preferably inhibiting, the endonuclease activity of the polypeptide fragment of the pa subunit, or variant thereof, of the present invention, and pharmaceutical compositions comprising said compounds for the treatment of conditions of disease caused by viral infections by the virus of the family orthomyxoviridae, family bunyaviridae and / or family arenviridae, preferably caused by viral infections by the pandemic influenza virus h1n1 2009. preferably, said compounds are identified by the methods disclosed herein, or said pharmaceutical compositions are produced by the methods disclosed here.fragmento de polipeptídeo, polinucleotídeo isolado, vetor recombinante, célula hospedeira recombinante, métodos para identificar compostos e para produzir compostos, anticorpo e uso do mesmo a presente invenção
