Systems and methods for localization of a puncture site on an atrial wall relative to a mammalian tissue of interest. In at least one embodiment, such a system includes a bodily access system, having an engagement catheter with an open distal end, a first lumen therethrough, and configured to reversibly attach to a first tissue using suction, a puncture device, configured to fit at least partially within the first lumen, and a scanner configured to identify at least a portion of the bodily access system when positioned within the luminal organ and further configured to identify a distance between a second tissue and a portion of the bodily access system. When such a system is used in connection with a therapeutic procedure, the distance between the second tissue and the portion of the bodily access system is used to facilitate a puncture of the first tissue at a desired location.
