<;P>;PROBLEM TO BE SOLVED: To provide a biological image acquisition apparatus capable of performing multi-directional and simultaneous observations and measurements in a short time and to provide a sample holding apparatus for implementing the biological image acquisition apparatus. <;P>;SOLUTION: A sample holding member 2 is supported by a supporting mechanism 12. The supporting mechanism 12 supports a pair of opposed end parts of the sample holding member 2. Gaps 14 are provided between unsupported end parts of the sample holding member 2 and the supporting mechanism 12. The gaps 14 are light-passing regions for passing reflected light from reflecting mirrors M3 and M4 on the back side of the sample holding member 2. <;P>;COPYRIGHT: (C)2010,JPO&INPIT