БАНШЕРО Жак Ф. (US),ЗУРАВСКИ Герард (US),ФЛАМАР Анне-Лаур (US),КОББ Аманда (US),МИД Холли (US),МОНТЕС Моника (US)
申请号：
RU2012153053/10
公开号：
RU2012153053A
申请日:
2012.12.10
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A method for increasing the effectiveness of antigen presentation of antigen presenting cell, comprising the steps of: a. provide preemphasis DC-specific antibody or functional fragment thereof to which is attached engineered Nef antigen where Nef antigen comprises optimizing the use of one or more codons that increases secretion of the antibody-antigen complex and b. brought into contact preassigned antigen-presenting cell with DC-specific antibody or functional fragment thereof under conditions where antibody-antigen complex and antigen are treated for T cell raspoznavaniya.2. A method according to claim 1 wherein the antigen presenting cell comprises a dendritic kletku.3. The method of claim 1, wherein the DC-specific antibody or functional fragment thereof binds to one half of the cohesin-dokerinovoy pairs and constructed Nef antigen is bound to the complementary half-cohesin dokerinovoy pair to form kompleksa.4. A method according to claim 1 wherein the antibody-antigen complex further comprises a flexible linker between the DC-specific antibody or functional fragment thereof and the Nef antigenom.5. A method according to claim 1 wherein the antibody-antigen complex further comprises a flexible linker between the DC-specific antibody or functional fragment thereof and the Nef antigen that comprises one or more glycosylation sites that provide increased flexibility between the antibody and the antigen, decreased proteolysis at the linker and sekretsiyu.6 increased. A method according to claim 1 wherein the antibody-antigen complex further comprises a flexible linker between the DC-specific antibody or a funct1. Способ повышения эффективности презентации антигена антигенпрезентирующей клеткой, включающий этапы, на которых:а. обеспечивают предварительно выделенное ДК-специфическое антитело или его функциональный фрагмент, к которому прикреплен сконструированный Nef антиген, где Nef антиген включает оптимизацию использования одного или более кодонов, что повышае
