Friesland Brands B.V.;Darling Ingredients Nederland B.V.;Rijksuniversiteit Groningen
发明人:
Erik Sallomons,Maarten Hotse Wilbrink,Peter Sanders,Johannis Paulus Kamerling,Catherina Anna van Vuure,Johannes Adrianus Hage
申请号：
US14363856
公开号：
US09539270B2
申请日:
2012.12.06
申请国别(地区):
US
年份:
2017
代理人:
摘要：
The invention relates to a method for providing analogs of human milk oligosaccharides (HMO), in particular oligosaccharides containing terminal sialic acid. The method comprises the steps of: a) providing a source of non-digestible galactooligosaccharides (GOS) containing at least two terminally bonded β-linked galactose residues； b) providing a sialic acid donor having (α2-3)-sialylated O-glycans； c) contacting said GOS with said sialic acid donor in the presence of an enzyme having trans-sialidase activity in an enzyme reaction mixture； and d) isolating from said enzyme reaction mixture a fraction comprising at least 20 percent by weight of disialylated galactooligosaccharides (di-Sia-GOS) based on the dry matter.
