A glaucoma testing device and a method of using the same are disclosed herein. The glaucoma testing device includes a device body portion having an outer housing and a flexible membrane at least partially defining a chamber supported within the outer housing; a fluid control system configured to insert an amount of the fluid into the chamber of the device body portion in order to expand the flexible membrane and exert pressure on an eye of a patient, or remove an amount of the fluid from the chamber of the device body portion in order to deflate the membrane and relieve pressure exerted on the eye; and a laser fiber optic configured to transmit a beam of light into the eye so as to produce a detectable ultrasonic wave so that an oxygenation level of the eye of the patient is capable of being determined using a photoacoustic system.