An ultrasonically pulsating high-pressure fluid jet coupled with a suction system serve as a tissue harvesting device. The pulsated fluid jet disrupts tissue, permitting both harvesting of cells for therapeutic delivery, and as a surgical dissector and aspirator, for liposuction, soft tissue dissection, etc. The jet is delivered to the target tissue through a cannula, coupled to an aspiration system. An ultrasonically actuated rod vibrates within the cannula engaging a nozzle, disrupting the fluid jet into droplets, permitting tuning of the jet to the resonant frequencies and impact pressures necessary to dissociate the target tissue. A suction recovery system may be separated or integrally formed with the fluid jet cannula, and is linked by a closed tubing system appropriate for sterilization and subsequent delivery of the harvested cells/tissues with or without growth factor or matrix addition for human re-implantation or for in vitro expansion for later re-implantation.
