#$%^&*AU2018201059A120180906.pdf#####ABSTRACT A method for detecting biological activity in a biological indicator comprises the steps of providing a known quantity of spores containing a fixed number of copies of an enzyme and a liquid growth medium comprising substrates of the enzyme. The enzyme substrates have a first emission spectrum, and are configured to be converted by the enzyme to substrate derivatives having a second emission spectrum. The spores are exposed to the liquid growth medium, and the liquid growth medium is measured for the second emission spectrum, and either no change in the second emission spectrum or a linear increase in the second emission spectrum, is detected as a function of time. -423536513vl6/6 914-" 1000 INCUBATE 1010 MONITOR FOR CHANGE IN FLUORESCENCE OF FLUID 1020 is THERE YES A LINEAR NO INCREASE IN FLUORESCENCE? 1030 1050 MONITOR FLUID FOR ASSUME STERILIZATION CHANGE TO EXPONENTIAL CYCLE EFFECTIVE INCREASE IN AND MEDICAL DEVICE FLUORESCENCE READY FOR USE 1040 YES CONFIRM STERILIZATION CYCLE INEFFECTIVE AND MEDICAL DEVICE NOT READY FOR USE MEDICAL DEVICE AND NEW BIOLOGICAL INDICATOR f106 0 SUBJECT TO STERILIZATION CYCLE (FIG.2) Fig. 6
