The present invention relates to a method for producing plasmacytoid dendritic cells having immune tolerance, plasmacytoid dendritic cells having immune tolerance produced by the method, and a cell therapeutic agent comprising the cells. The present invention can induce a high yield of plasmacytoid dendritic cells having immune tolerance from immature dendritic cells through a simple and easy process, thereby enabling a stable supply of the plasmacytoid dendritic cells having immune tolerance in large quantities.