BHK-21 Novel BHK-21 Cell Line Available for Suspension Culture in Serum-Free Medium and Method for Foot-Mouth Disease Vaccine Production Using the Same
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY;REPUBLIC OF KOREA(ANIMAL AND PLANT QUARANTINE AGENCY)
发明人:
LEE, EUN GYO,이은교,PARK, SOON YONG,박순용,TARK, DONG SEOB,탁동섭,LEE, HYANG SIM,이향심,KO, YOUNG JOON,고영준,KIM, YEON GU,김연구,PARK, SUN YOUNG,박선영,KIM, CHUNG SU,김충수,RYU, KYOUNG HWA,류경화,LEE, MYOUNG HEON,이명헌,LEE, EUN GYOKR,PARK, SOON YONGKR,TARK, DONG SEOBKR,LEE, HYANG SIMKR,KO, YOUNG JOONKR,KIM, YEON GUKR,PARK, SUN YOUNGKR,KIM, CHUNG SUKR,RYU, KYOUNG HWAKR,LEE, MYOUNG
申请号：
KR1020170101078
公开号：
KR1018122230000B1
申请日:
2017.08.09
申请国别(地区):
KR
年份:
2017
代理人:
摘要：
The present invention relates to a novel BHK-21 cell line available for suspension culture in a serum-free medium and to a method for manufacturing a foot-and-mouth disease vaccine using the same, wherein a novel BHK-21 cell could be suspension-cultured under serum-free conditions and the virus productivity was also increased compared to wild-type BHK-21 cells, and thus it is possible to utilize the novel BHK-21 cells of the present invention for the manufacture of various useful substances such as viruses, proteins, peptides and the like.본 발명은 무혈청 배지에서 현탁 배양이 가능한 신규 BHK-21 세포주 및 이를 이용한 구제역 백신 제조방법에 관한 것으로, 본 발명의 신규 BHK-21 세포는 혈청이 없는 조건에서 현탁 배양이 가능하고 바이러스 생산성 또한 야생형 BHK-21 세포에 비해서 증가하였으므로, 본 발명의 신규 BHK-21 세포를 이용하여 백신용 바이러스, 단백질, 펩타이드 등 다양한 유용물질의 생산에 활용될 수 있다.
