MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V.
发明人:
REEVES, Guy,REED, Floyd
申请号：
EPEP2013/077856
公开号：
WO2014/096428A1
申请日:
2013.12.20
申请国别(地区):
EP
年份:
2014
代理人:
摘要：
The present invention relates to a method for reducing the competitive fitness of an organism hemizygous for a transgenic locus compared to the organism homozygous for the transgenic locus comprising the steps of:(a) reducing the expression of a haploinsufficient gene in the organism, wherein said reduction is conveyed by a transgenic locus in the organism； and (b) rescuing the reduced expression in the organism, wherein said rescue is conveyed by the same transgenic locus in the organism, yielding an organism which is less competitively fit if hemizygous for the transgenic locus than if homozygous for the transgenic locus. The present invention also relates to a method for decreasing the introgression of a transgenic locus in an organism into a population of otherwise interfertile sexually reproducing organisms comprising the steps of: (a) reducing the expression of a haploinsufficient gene in the organism, wherein said reduction is conveyed by a transgenic locus in the organism； (b) rescuing the reduced expression in the organism, wherein said rescue is conveyed by the same transgenic locus in the organism, and (c) using a transgenic organism obtained in step (b) in an environment comprising otherwise interfertile sexually reproducing wildtype individuals of the organism, wherein the competitive fitness of hemizygous progeny is reduced, thereby decreasing the rate of sexually reproduction and/or viability and/or the competitive fitness of hemizygous progeny. Further envisaged are corresponding genetic systems and genetically modified organisms.La présente invention concerne un procédé destiné à réduire l'aptitude compétitive d'un organisme hémizygote pour un locus transgénique par comparaison à l'organisme homozygote pour le locus transgénique, le procédé comportant les étapes consistant à: (a) réduire l'expression d'un gène haploinsuffisant dans l'organisme, ladite réduction étant véhiculée par un locus transgénique dans l'organisme； et (b) préserver l'expression
