Use of NADPH for preparation of a drug for antiplatelet aggregation, according to the research, it is found that in-vitro administration of exogenous NADPH inhibits ADP-induced platelet aggregation of rats in a dose-dependent mode in-vitro administration of exogenous NADPH inhibits Thrombin-induced platelet aggregation of rats in a dose-dependent mode preventive administration of NADPH in the rats can remarkably inhibit ADP-induced platelet aggregation preventive administration of NADPH in the rats can remarkably inhibit Thrombin-induced platelet aggregation. Therefore, NADPH has a platelet aggregation inhibition function and can be used as a potential drug for antiplatelet aggregation.