This invention is a methodology for the long-term augmentation, and/or repairof dermal, subcutaneous, or vocal cord tissue by the injection, or directsurgical placement of autologous cultured fibroblasts derived from connectivetissue, or dermis, or fascia lamina propria tissue fibroblasts derived fromthe lamina propria or adipocytes. The fibroblast cultures utilized for theaugmentation, and/or repair of skin defects are derived from either connectivetissue, dermal, and/or fascial fibroblasts. In addition a methodology ofrendering the cultured cells substantially free of potentially immunogenicserum derived proteins by late stage passage of the cultured fibroblastslamina propria tissue, or adipocytes in serum free medium in the patients ownserum.
