The present disclosure provides a chimeric non-human animal comprising human hepatocytes, methods for preparing the chimeric non-human animal comprising human hepatocytes and methods of utilizing the chimeric non-human animal comprising human hepatocytes to screening and identifying metabolites for any type of drugs, typically small molecule drugs, which might affect human liver functions and any other bodily function.本發明提供包含人類肝細胞之嵌合非人類動物、用於製備該包含人類肝細胞之嵌合非人類動物之方法,及使用該包含人類肝細胞之嵌合非人類動物以篩選及識別可影響人類肝功能及任何其他身體功能之任何類型的藥物(通常係小分子藥物)之代謝產物之方法。