The present invention relates to an ultrasound calibration device comprising a body portion having at least one echogenic fiducial; a marker portion having at least one tracking marker which can be detected by a medical tracking system; and a hook-shaped mounting portion extending from the body portion.The present invention also relates to a method for calibrating an ultrasound probe, comprising the steps of filling a container with a fluid, in particular a physiologic salt solution; placing an ultrasound calibration device in accordance with the invention into the container; comparing, with the aid of a medical navigation system, a calculated position of at least one fiducial with a determined position of the at least one fiducial which is determined using a tracked ultrasound probe.