HADASIT MEDICAL RESEARCH SERVICES & DEVELOPMENT LIMITED
发明人:
KARUSSIS, DIMITRIOS,KASSIS, IBRAHIM
申请号：
CA2926575
公开号：
CA2926575A1
申请日:
2014.10.07
申请国别(地区):
CA
年份:
2015
代理人:
摘要：
The present disclosure concerns a method of inducing transdifferentiation of mesenchymal stem cells (MSC), the method comprising (a) culturing MSC in a first culture medium comprising a growth factor selected for allowing formation of neuralized MSC (NMSC)； (b) allowing the NMSC to proliferate for a sufficient time during which said culture medium is renewed at least once； and (c) culturing the NMSC of (b) in a second culture media comprising cerebrospinal fluid (CSF) for a time sufficient for the NMSC to differentiate into a population of cells comprising terminally differentiated neurons, astrocytes and oligodendrocytes. Also provided by the present invention is the use of MSC or NMSC for providing a composition comprising said population and to kits comprising MSC or NMSC and instructions for use of same.
