PURPOSE: A method for determining a variety with resistance to tomato yellow leaf curl virus (TYLCV) is provided to enhance convenience and precision in resistivity evaluation and to cheaply and repetitively evaluate resistance. CONSTITUTION: A method for determining a variety with resistance to TYLCV comprises the step of infecting plant leaves with TYLCV strains. The method also comprises the step of measuring TYLCV-Ct/Actin-Ct value. The method additionally comprises the step of determining a resistant variety when the TYLCV-Ct/Actin-Ct value is 0.7 or greater. TYLCV includes at least three among TYLCV-IS, TYLCV-NS, TYLCV-YS, TYLCV-GJ, and TYLCV-Cgh. [Reference numerals] (AA) Bacchus; (BB) Supper sunroad; (CC) Ten ten본 발명은 TYLCV에 대한 저항성 품종을 판정하는 방법에 관한 것으로, 본 발명에 따른 판정 방법을 이용할 경우, 저항성 평가의 편리성 및 정밀성이 증대되고, 분리주의 보전이 용이하여 적은 비용으로 반복적으로 평가할 수 있다.