Formation manner of the skeletal muscle cell which includes the fact that this invention, concretely, culturing the multipurpose Characteristic main stem cell of mammal origin with the BMP4 content serum-free medium, induces the PDGFR positive mesoderm precursive cell from the multipurpose Characteristic main stem cell, the osteoclast, in regard to the manner which it differentiates induces the cartilage cell or the muscular cell in the bloodless clearly nutrient medium, to the osteoclast or cartilage cell after culturing manner, and the multipurpose Characteristic main stem cell which differentiate with the BMP4 content serum-free medium, bloodless under BMP4 non existing and LiCl existence cultures clearly with the nutrient medium and to the PDGFR positive skeletal muscle precursive cell differentiates inducesIt regards.この発明は、多能性幹細胞から、無血清培地中で骨細胞、軟骨細胞又は筋肉細胞を分化誘導する方法に関し、具体的には、哺乳動物由来の多能性幹細胞をBMP4含有無血清培地で培養してPDGFRα陽性中胚葉前駆細胞を誘導し、骨細胞又は軟骨細胞へ分化する方法、並びに、多能性幹細胞をBMP4含有無血清培地で培養したのち、BMP4非存在、LiCl存在下の無血清培地で培養しPDGFRα陽性骨格筋前駆細胞へ分化誘導することを含む骨格筋細胞の形成方法に関する。
