Provided in the present invention is a method for initiating the proliferation, migration and differentiation of stem cells in mammals, comprising the following steps: preparing a stem cell initiation agent containing chlorine dioxide and administering the stem cell initiation agent to target tissues of mammals, wherein an effective amount of chlorine dioxide is provided when administering the stem cell initiation agent to the target tissues of mammals. Also provided in the present invention is the use of chlorine dioxide in the preparation of a drug for initiating the proliferation, migration and differentiation of stem cells. The method of the present invention has a broad application range, and is suitable for various requirements of treating diseases using the tissue regeneration ability and immunity regulation ability of stem cells or prompting the initiation of the proliferation, migration and differentiation of stem cells in injured organs and organisms having regenerated tissues； furthermore, using the method of the present invention, the proliferation, migration and differentiation of stem cells can be initiated quickly and moderately； and the method has few side effects or no side effects, and can continuously be used for initiating the proliferation, migration and differentiation of stem cells.L'invention concerne une méthode permettant de déclencher la prolifération, la migration et la différenciation de cellules souches chez un mammifère, ladite méthode consistant à : préparer un agent d'initiation des cellules souches contenant du dioxyde de chlore, et administrer l'agent à des tissus cibles chez un mammifère, la quantité de dioxyde de chlore efficace étant apportée lors de cette administration. L'invention concerne également l'utilisation du dioxyde de chlore dans la préparation d'un médicament permettant d'initier la prolifération, la migration et la différenciation de cellules souches. La méthode selon l'invention présente un large éventail d'applic
