The present invention relates to a method and a system for the production of arbuscular mycorrhizal fungi (AMF). More specifically the invention described herein relates to a method and a system for the continuousculture of arbuscular mycorrhizal fungi preferably including steps of harvesting and/or passaging. Further the invention relates to culture conditions suitable for said continuous cultivation method and system. Another aspect of the invention is a sterile inoculum comprising AMF colonized root material and other propagules like AMF spores. Yet another aspect of the invention relates to the use of AMF colonized root material for continuous production of arbuscular mycorrhizal fungi material in liquid culture medium.
