Various surgical kits are provided including a sealing cuff configured to be positioned around a body lumen and a sealant. In one embodiment the sealing cuff can form an enclosed loop around an anastomosis and can define an interior chamber for receiving sealant therein. The sealing cuff can ensure that sealant remains in contact with the body lumen as the sealant cures and reinforces the anastomosis. Methods for sealing an anastomosis are also provided and include delivering sealant to the sealing cuff inserting inflatable members so that they are positioned adjacent to the anastomosis and delivering fluid to at least one of the inflatable members to expand an inner wall of the tubular organ. In certain aspects gas and/or liquid can be delivered to an anastomosis such as after the sealant has solidified in order to test the effectiveness of the seal.