THE UNIVERSITY OF MANCHESTER;GARLAND, JOHN, MICHAEL
发明人:
GARLAND, JOHN, MICHAEL
申请号：
WO2004GB01821
公开号：
WO2004101735(A3)
申请日:
2004.04.28
申请国别(地区):
世界知识产权组织国际局
年份:
2005
代理人:
摘要：
A method and device for culturing tissue, in particular microvascularized tissue, to aid tissue graft survival by establishing a network of small vessels that can be linked to the host arterial and venous supplies. A scaffold (2) seeded with the cells destined to be the tissue is provided with an upper guide layer (4) having a pattern of apertures (6) therethrough and vascular endothelial cells (5) are delivered to the layer. The scaffold is provided with a chemoattractant for the vascular endothelial cells, such as vascular endothelial growth factor, and fluid is passed over the guide layer. The apertures allow fluid and vascular endothelial cells to migrate into the scaffold only at these focal points to generate capillary sprouts, the migration of cells being stimulated by the chemoattractant within the scaffold.
