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СОСТАВ АНТИГЕНОВ ВИРУСА ГЕПАТИТА В ДЛЯ КЛЕТОЧНОЙ СТИМУЛЯЦИИ С ПОСЛЕДУЮЩЕЙ ТЕРАПЕВТИЧЕСКОЙ ИММУНИЗАЦИЕЙ
专利权人:
СЕНТРО ДЕ ИНХЕНЬЕРИЯ ХЕНЕТИКА И БИОТЕКНОЛОХИЯ
发明人:
АГИЛАР РУБИДО Хулио,ЛОБАЙНА МАТО Ядира,ЭРНАНДЕС ИНГВАНСО Дунья,ТРУХИЛЬО ПЕРЕС Эиди,ФРЕЙРЕ АЛЬМЕИДА Фрейя де Лос Милагрос,МУСИО ГОНСАЛЕС Верена Лусила,ПЕНТОН АРИАС Эдуардо,ГОНСАЛЕС БЛАНКО Сонья,УБЬЕТА
申请号:
RU2012117233
公开号:
RU2012117233A
申请日:
2010.09.28
申请国别(地区):
RU
年份:
2013
代理人:
摘要:
1. Structure of the surface (HBsAg) and the nucleocapsid (HBcAg) antigens of hepatitis B virus B (HBV), characterized in that it contains said antigens precipitates in suspension.2. Composition according to claim 1, wherein the precipitates in the suspension mixture composed of particles of size less than 500 nm and 500 nm.3. The composition of claim 2, wherein the particle size of less than 500 nm, and 500 nm are in the ratio from 50-50% to 80-20%, respectively.4. The composition of claim 1, wherein the precipitates in the suspension obtained by subjecting the mixture of the acid deposition process antigens.5. The composition of claim 4 wherein acid precipitation is achieved by dialysis or diafiltration against acid.6. A process for preparing the composition of the surface (HBsAg) and the nucleocapsid (HBcAg) of hepatitis B virus antigens B (HBV), said antigens formed precipitates in the suspension, characterized in that: a) a mixture and HbcAg HbsAg antigen, b) subjecting the mixture of the acid process deposition, and c) precipitates is selected in accordance with their size so that they provide maximum in vivo or in vitro stimulation of autologous or heterologous antigen-presenting cells.7. The method of claim 6, wherein the concentration of the HbsAg and HbcAg antigens in the mixture, which was subjected to the acid deposition process is from 0.1 to 2 mg / ml protein.8. The method of claim 6, wherein the acid precipitation achieved by diafiltration or dialysis against acid.9. The method of claim 8, wherein the acid solution is a dialysis or diafiltration is acetic acid, preferably 50 mM acetic acid, pH 4,0.10. The method of claim 8, wherein the acid deposition continues for a period of from 10 minutes to 24 hours.11. The method of claim 6, wherein the precipitate particle size is selected so that the particle size of less than 500 nm, and 500 nm are in the ratio from 50-50% to 80-20%, respectively.12. A method for stimulating cell passive immunotherapy, charact
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