A device for staining suspended cells composed of a cannula (1), a pushrod (2), a plunger sealing pad (3), and a capture membrane (4), wherein the plunger sealing pad (3) is located at an upper part within the cavity of the cannula (1), the capture membrane (4) is located at a lower part within the cavity of the cannula (1), and an opening (5) above the membrane and an opening (6) below the membrane are formed on the wall of the cannula (1) respectively. The device is for use in staining suspended cells, is capable of effectively overcoming the drawbacks in current existing methods for staining cells, such as high failure rates, low accuracy and repeatability, and being very time-consuming, etc., and has advantages such as high success rates, good accuracy and repeatability, and not being time-consuming, etc.