PURPOSE: A biological membrane permeable composition is provided to produce a carrying object substance with high vitality in high yield rate, and a composition with low molecular weight protamine combined to one end or both ends. CONSTITUTION: A biological membrane permeable composition includes a carrying object substance, and low molecular weight protamine. The low molecular weight protamine is combined to one or both ends of the carrying object substance, and has an amino acid sequence which has over 70% of homology to one or more of amino acid sequence with the amino acid sequence number 1 and the amino acid sequence number 2. The carrying object substance includes one or more selected from the group consisting of protein, polypeptide, peptide, nucleoacid, mRNA, and antisense RNA. The protein includes one selected from DKK-2(Dickkopf-related protein-2), adipokine, thrombospondin, and superoxide dismutase. The carrying object substance is one or more selected from growing factors, enzymes, hormones, transcription factors, toxin, antigen, and antibody. [Reference numerals] (AA) Concentration in cells(uM)본 발명은 운반 대상 물질； 및 운반 대상 물질의 한쪽 또는 양쪽 말단에 결합된 서열 번호 1 또는 서열 번호 2의 아미노산 서열을 포함하는 저분자량 프로타민을 포함하는 생체막 투과성 조성물을 개시한다.
