THIS INVENTION RELATES TO THE FIELD OF IMMUNOLOGY AND MORE SPECIFICALLY RELATES TO ANTI-PHYTASE MONOCLONAL ANTIBODIES. IMMUNOLOGY METHODS, KITS, AND REAGENTS, FOR THE DETECTION OF A PHYTASE FROM OR DERICED FROM E. COLI PHYTASE, IN PARTICULAR, QUANTUMTM PHYTASE. THE INVENTION FURTHER RELATES TO HYBRIDOMA CELL LINES THAT PRODUCE ANTI-PHYTASE MONOCLONAL ANTIBODIES.